Antibody Screening Platforms

The discovery of antibodies with desired characteristics, such as high affinity and specificity, is a crucial step in harnessing their full potential. Leveraging our team's vast expertise, we have developed robust antibody screening platforms that offer a wide array of services to facilitate the discovery and development of single domain antibodies (sdAbs).

Purpose of Single Domain Antibody Screening

Single domain antibody (sdAb) screening serves the primary purpose of identifying sdAbs that bind specifically to a target antigen of interest. This process involves screening large libraries of antibody candidates to identify those with the desired binding properties. Antibody screening platforms enable the identification of sdAbs with high affinity, specificity, and functional characteristics, making them invaluable tools in research, diagnostics, and therapeutics.

The whole process of antibody screening. Fig. 1 Schematic overview of B cell sorting. (Anand T, et al., 2021)

Platforms for Single Domain Antibody Screening

Multiple methods are employed in the screening of single domain antibodies, including phage surface display, yeast surface display, bacterial surface display, mammalian cell surface display, ribosome and mRNA display, B cell sorting, etc.

Platforms	Advantages	Disadvantages
Phage Surface Display	High-throughput screening Ability to screen large libraries of sdAbs Ease of manipulation and engineering of phage particles Well-established and widely used method Strong compatibility	Limitation in the size of sdAbs that can be displayed Possibility of phage particle instability and aggregation The diversity of phage libraries depends on bacterial transformation efficiency. High cost
Yeast Surface Display	Ability to express complex eukaryotic proteins Ability to perform protein-protein interaction studies High affinity screening	Limited diversity compared to phage display Potential for glycosylation and other post-translational modifications in yeast cells Challenges in scaling up production for large-scale applications
Bacteria Surface Display	Simplicity and ease of use Fast and efficient screening of sdAb libraries Strong compatibility High expression levels and rapid production	Limited diversity compared to other display methods Potential for limited stability and solubility of sdAbs Challenges in displaying complex antibodies
Mammalian Cell Surface Display	Ability to display full-length IgG antibodies on the cell surface Mimics the native antibody structure and function Enables screening for functional antibodies Suitable for applications requiring post-translational modifications	Complex and time-consuming process Limited library diversity compared to other methods Challenges in maintaining cell viability and stability during the screening process
Ribosome and mRNA Display	Cell-free system High diversity libraries sdAbs with improved stability and solubility Strong compatibility	Complex and technically demanding process Challenges in optimizing translation efficiency and library quality
B Cell Sorting	Enables direct identification of sdAbs from B cells Captures the natural diversity of the immune repertoire	Rely on the availability of B cells from immunized animals or patients Time-consuming and labor-intensive process Limited control over the diversity and specificity of the obtained sdAbs

Our Services

Single domain antibody (sdAb) preparation and screening represent a groundbreaking advancement in the quest for novel therapeutics. As a pioneering company at the forefront of this field, we have established the fully human single domain antibody platform and antibody screening platforms that are specifically designed to drive the preparation, discovery and development of sdAbs. Our platforms harness a diverse range of state-of-the-art antibody preparation and screening technologies, allowing us to cater to your specific research requirements.

Our Platforms for Single Domain Antibody Screening

Phage Surface Display

Our phage surface display platform enables high-throughput screening, allowing you to select sdAbs with exceptional affinity and specificity for your target of interest.

Yeast Surface Display

With its direct screening capabilities and compatibility with high-throughput screening, yeast surface display streamlines your antibody discovery process.

Bacteria Surface Display

Our user-friendly and efficient platform leverages the simplicity of bacteria to rapidly screen and produce sdAbs tailored to your needs.

Mammalian Cell Surface Display

With mammalian cell surface display, you can explore applications requiring post-translational modifications and unlock the potential for therapeutic breakthroughs.

Ribosome and mRNA Display

Our cell-free system bypasses the need for intracellular antibody expression, enabling efficient screening and selection of diverse sdAbs.

B Cell Sorting

Our cutting-edge technology allows for the direct identification and isolation of sdAbs from B cells, providing a valuable tool for your therapeutic research.

Our Advantages

Time-saving services with high efficiency

Professional and experienced team

Cutting edge technology platform

Numerous service cases and customer praise

At our company, we are dedicated to pushing the boundaries of single domain antibody screening technologies. We are committed to driving innovation and developing novel approaches that revolutionize the field. If you are interested in our services, please don't hesitate to contact us for further information and pricing details.

References

Han X, Wang Y, Li S, et al. A rapid and efficient screening system for neutralizing antibodies and its application for SARS-CoV-2[J]. Frontiers in immunology, 2021, 12: 653189.
Ojima-Kato T, Nagai S, Nakano H. Ecobody technology: rapid monoclonal antibody screening method from single B cells using cell-free protein synthesis for antigen-binding fragment formation[J]. Scientific Reports, 2017, 7(1): 13979.

For research use only, not for clinical use.

Platforms

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