B cell sorting has revolutionized antibody discovery and development. Recognizing the enormous potential of this technology, our scientists have established a B cell sorting platform. By leveraging this platform and our collective expertise, we are able to efficiently screen and isolate high-quality single domain antibodies to meet your diagnostic and therapeutic development needs.

What is B Cell Sorting?

B cell sorting is a powerful tool for screening and isolating high affinity antibodies. The method typically begins by isolating B cells from human peripheral blood or the spleen of immunized animals. These B cells are then subjected to deep sequencing and single-cell analysis to develop potential optimized antibody candidates.

Fig. 1 Schematic representation of the single-B cell sorting protocol used for antibody discovery from immunized mice. (Starkie D O, et al., 2016)

Our Services

With our company's prominent position in the field of antibody discovery and development, we have joined forces with industry-leading experts to create a cutting-edge B cell sorting platform. This platform has been specifically designed to expedite the process of discovering and screening single domain antibodies (sdAbs) for a diverse range of therapeutic and diagnostic applications.

Workflow of B Cell Sorting

Immunization

During immunization, we administer antigens to experimental animals to stimulate the activation and proliferation of B cells. These B cells undergo a process called affinity maturation, which leads to the production of high-affinity antibodies.

B Cell Isolation

We isolate B cells from the spleen of immunized animals using either fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS) techniques. Isolating B cells ensures that the subsequent analysis focuses specifically on the antibody-producing cells.

Deep Sequencing

Subsequently, we employ deep sequencing and single-cell analysis techniques to decipher the genetic information encoded within the B cell receptor (BCR). This helps us gain insight into the antibody repertoire and identify potential antibody candidates.

Antibody Optimization

The selected sdAbs can be further optimized through affinity maturation or engineering to improve their binding properties and other desirable traits. Optimized sdAbs can be produced at a large scale using recombinant protein expression systems.

Antibody Characterization

The isolated antibodies undergo rigorous characterization to assess affinity, specificity, and functionality. We use ELISA, surface plasmon resonance (SPR), and flow cytometry to assess antibody binding kinetics, target specificity, and epitope recognition.

Antibody Expression

The process involves amplifying and cloning the genes encoding the variable regions of the antibody into an expression vector to produce recombinant antibodies. Commonly used expression systems include mammalian cell lines and yeast.

Advantages of B Cell Sorting

Our Advantages

Through the integration of cutting-edge technologies, our company continues to drive advancements in antibody discovery, offering tailored solutions for various therapeutic challenges. If you are interested in our services, please don't hesitate to contact us for further information and pricing details.